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A high-throughput method to identify trans-activation domains within transcription factor sequences.

Arnold CD, Nemcko F, Woodfin AR, Wienerroither S, Vlasova A, Schleiffer A, Pagani M, Rath M, Stark A
EMBO J. 2018 Aug 15;37(16). pii: embj.201798896. doi: 10.15252/embj.201798896. Epub 2018 Jul 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
111930ptAD-seq-ubi63E-Gal4-DBDVector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.
111931pGL4.26_4xUAS_DSCP_GFPVector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Antibodies from Article