p62 filaments capture and present ubiquitinated cargos for autophagy.
Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S
EMBO J. 2018 Mar 1;37(5). pii: embj.201798308. doi: 10.15252/embj.201798308. Epub 2018 Jan 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169168 | 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B) | Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction. |
| 190191 | pET-Duet1-His-TEV-mCherry-OPTN WT | Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797 |
| 190192 | pET-Duet1-6xHis-TEV-OPTN | Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397 |
| 190194 | pET-Duet1-6xHis-TEV-NDP52 | Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401 |