Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing.
Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y
Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120553 | ScFv-2ERT2-V | 3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64. |
| 120555 | EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo | Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells |
| 120556 | ScFv-2ERT2-VPH | Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1 |