Engineering Nanodisc Scaffold Proteins for Native Mass Spectrometry.

Reid DJ, Keener JE, Wheeler AP, Zambrano DE, Diesing JM, Reinhardt-Szyba M, Makarov A, Marty MT
Anal Chem. 2017 Nov 7;89(21):11189-11192. doi: 10.1021/acs.analchem.7b03569. Epub 2017 Oct 23. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
97323	pMSP1D1R1	Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
97325	pMSP1D1G1	Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
97327	pMSP1D1T1	Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
97328	pMSP1D1T2	Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.