CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level.
Yuen G, Khan FJ, Gao S, Stommel JM, Batchelor E, Wu X, Luo J
Nucleic Acids Res. 2017 Nov 16;45(20):12039-12053. doi: 10.1093/nar/gkx843.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164929 | LentiCRISPRv2 NT sgRNA3 | Expresses Non-targeting sgRNA3 control in mammalian cells |
| 164930 | LentiCRISPRv2 NT sgRNA5 | Expresses Non-targeting sgRNA5 control in mammalian cells |
| 164932 | LentiCRISPRv2 EGFP sgRNA1 | Expresses EGFP sgRNA1 in mammalian cells |
| 164933 | LentiCRISPRv2 EGFP sgRNA2 | Expresses EGFP sgRNA2 in mammalian cells |
| 164934 | LentiCRISPRv2 EGFP sgRNA4 | Expresses EGFP sgRNA4 in mammalian cells |