Modular fluorescence complementation sensors for live cell detection of epigenetic signals at endogenous genomic sites.
Lungu C, Pinter S, Broche J, Rathert P, Jeltsch A
Nat Commun. 2017 Sep 21;8(1):649. doi: 10.1038/s41467-017-00457-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191392 | Flag-NLS-MaSat ZF-18-mVenusN | Zinc finger fused to N-terminal part of split mVenus |
| 191393 | pcDNA3 Flag-MBD1-14-mVenusC | MBD domain of MBD1 fused to C-terminal part of split mVenus |
| 191394 | pcDNA3 Flag-MBD1 R44Q-14-mVenusC | R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus |
| 191395 | pTH-PanCen TALE-mVenusN | TALE fused to N-terminal part of split mVenus |
| 191396 | pHAGE-TO-Sp dCas9-mVenusN | SpdCas9 fused to N-terminal part of mVenus |
| 191397 | pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo | sgRNA targeting alpha-satellites on human chromosome 9 |
| 191398 | FLAG-mVenusC-14-HP1b Chromo | HP1b chromo domain fused to C-terminal part of split mVenus |
| 191399 | FLAG-mVenusC-14-HP1b Chromo W42A | W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus |