Purification of metal-dependent lysine deacetylases with consistently high activity.
Toro TB, Painter RG, Haynes RA, Glotser EY, Bratton MR, Bryant JR, Nichols KA, Matthew-Onabanjo AN, Matthew AN, Bratcher DR, Perry CD, Watt TJ
Protein Expr Purif. 2018 Jan;141:1-6. doi: 10.1016/j.pep.2017.08.009. Epub 2017 Aug 24.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
224251 | pJExpress401-KDAC4 CD | Expresses human KDAC4 (HDAC4) catalytic domain in E. coli |
224341 | pJExpress401-KDAC7 CD | Expresses human KDAC7 (HDAC7) catalytic domain in E. coli |
224342 | pJExpress401-KDAC8 H143A | Expresses human KDAC8 (HDAC8) H143A in E. coli |
224344 | pFastBacI-KDAC8 H143A | Expresses human KDAC8 (HDAC8) H143A in insect cells |
224345 | pFastBacI-KDAC7 CD | Expresses human KDAC7 (HDAC7) catalytic domain in insect cells |
224346 | pFastBacI-KDAC6 | Expresses human KDAC6 (HDAC6) in insect cells |