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Purification of metal-dependent lysine deacetylases with consistently high activity.

Toro TB, Painter RG, Haynes RA, Glotser EY, Bratton MR, Bryant JR, Nichols KA, Matthew-Onabanjo AN, Matthew AN, Bratcher DR, Perry CD, Watt TJ
Protein Expr Purif. 2018 Jan;141:1-6. doi: 10.1016/j.pep.2017.08.009. Epub 2017 Aug 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
224251pJExpress401-KDAC4 CDExpresses human KDAC4 (HDAC4) catalytic domain in E. coli
224341pJExpress401-KDAC7 CDExpresses human KDAC7 (HDAC7) catalytic domain in E. coli
224342pJExpress401-KDAC8 H143AExpresses human KDAC8 (HDAC8) H143A in E. coli
224344pFastBacI-KDAC8 H143AExpresses human KDAC8 (HDAC8) H143A in insect cells
224345pFastBacI-KDAC7 CDExpresses human KDAC7 (HDAC7) catalytic domain in insect cells
224346pFastBacI-KDAC6Expresses human KDAC6 (HDAC6) in insect cells

Antibodies from Article