Voltage-gated calcium flux mediates Escherichia coli mechanosensation.
Bruni GN, Weekley RA, Dodd BJT, Kralj JM
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9445-9450. doi: 10.1073/pnas.1703084114. Epub 2017 Aug 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98917 | pKL001 | Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli. |
| 98919 | pKL003 | Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli. |
| 98920 | pKL004 | Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli. |
| 98921 | pKL002 | pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS. |