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Plk1 regulates contraction of postmitotic smooth muscle cells and is required for vascular homeostasis.

de Carcer G, Wachowicz P, Martinez-Martinez S, Oller J, Mendez-Barbero N, Escobar B, Gonzalez-Loyola A, Takaki T, El Bakkali A, Camara JA, Jimenez-Borreguero LJ, Bustelo XR, Canamero M, Mulero F, de Los Angeles Sevilla M, Montero MJ, Redondo JM, Malumbres M
Nat Med. 2017 Aug;23(8):964-974. doi: 10.1038/nm.4364. Epub 2017 Jul 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136325EGFP-Mm_MyoGEF(WT)Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP
136327pENTR-Mm_MyoGEF(WT)Mouse MyoGEF-WT in Gateway pENTR
136328pENTR-Mm_MyoGEF(T575A)Mouse MyoGEF-T575A mutant in Gateway pENTR
136330pLENTI_Hu_ECT2(WT)Lentiviral expression of human ECT2 WT
136332pLENTI_Hu_ECT2(T327D)Lentiviral expression of human ECT2_T327D
136335pDEST-V5_Hu_p115-RhoGEF(WT)Mammalian expression of human p115-RhoGEF with N-terminal V5
136337pDEST-EGFP_Hu_p115-RhoGEF(WT)Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Antibodies from Article