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Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation.

Tang W, Hu JH, Liu DR
Nat Commun. 2017 Jun 28;8:15939. doi: 10.1038/ncomms15939. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
96849pWT016gprocessed HHR-sgRNA-GFP
96850pWT016fHHR-bsgRNA-GFP (10 nt blocker)
96851pWT016edHHR-bsgRNA-GFP (10 nt blocker)
96852pWT016aHHR-bsgRNA-GFP (13 nt blocker)
96853pWT016bdHHR-bsgRNA-GFP (13 nt blocker)
96854pWT016hHHR-bsgRNA-GFP (17 nt blocker)
96855pWT016ddHHR-bsgRNA-GFP (17 nt blocker)
96856pWT016itheophylline-agRNA-GFP
96857pWT016l(d)theophylline-agRNA-GFP
96858pWT029cguanine-agRNA (GFP activation)
96859pWT029d(d)guanine _agRNA (GFP activation)
96860pWT029gguanine-agRNA (GFP activation, 18 nt blocker with bulges)
96861pWT029h(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)
96862pWT049dguanine-agRNA (19 nt blocker with bulges, RFP activation)
96863pWT049e(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)
96864pWT055gsgRNA-HEK3
96865pWT055fsgRNA-FANCF
96866pWT055esgRNA-EMX1
96867pWT055hsgRNA-HEK4
96868pWT057eHHR-bsgRNA-HEK3
96869pWT057fdHHR-bsgRNA-HEK3
96870pWT057cHHR-bsgRNA-FANCF
96871pWT057ddHHR-bsgRNA-FANCF
96872pWT057aHHR-bsgRNA-EMX1
96873pWT057bdHHR-bsgRNA-EMX1
96874pWT057gHHR-bsgRNA-HEK4
96875pWT057hdHHR-bsgRNA- HEK4
96876pWT055ctheophylline-agRNA-HEK3
96877pWT055btheophylline-agRNA-FANCF
96878pWT015aGFP reporter
96879pWT032aRFP reporter

Antibodies from Article