Skin-specific regulation of SREBP processing and lipid biosynthesis by glycerol kinase 5.
Zhang D, Tomisato W, Su L, Sun L, Choi JH, Zhang Z, Wang KW, Zhan X, Choi M, Li X, Tang M, Castro-Perez JM, Hildebrand S, Murray AR, Moresco EMY, Beutler B
Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5197-E5206. doi: 10.1073/pnas.1705312114. Epub 2017 Jun 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 134257 | pBOB-C-Flag-GK5_v1 | Lentivector encoding Flag-tagged GK5 (isoform 1) |
| 134260 | pBOB-C-Flag-GK5_v2 | Lentivector encoding Flag-tagged GK5 (isoform 2) |
| 134261 | pBOB-C-Flag-GK5-toku (1-174) | Lentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174) |
| 134262 | pBOB-C-Flag-GK5 (1-287) | Lentivector encoding Flag-tagged GK5 truncation (amino acids 1-287) |
| 134263 | pBOB-C-Flag-GK5 (25-287) | Lentivector encoding Flag-tagged GK5 truncation (amino acids 25-287) |
| 134264 | pBOB-C-Flag-GK5 (287-534) | Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-534) |
| 134265 | pBOB-C-Flag-GK5 (287-396) | Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-396) |
| 134266 | pBOB-C-Flag-GK5 (396-534) | Lentivector encoding Flag-tagged GK5 truncation (amino acids 396-534) |
| 134267 | pBOB-C-Flag-GK5 (416-534) | Lentivector encoding Flag-tagged GK5 truncation (amino acids 416-534) |
| 134268 | pBOB-C-3HA-GK5_v1 | Lentivector encoding 3XHA-tagged GK5 (isoform 1) |
| 134269 | pBOB-C-3HA-GK5_v2 | Lentivector encoding 3XHA-tagged GK5 (isoform 2) |
| 134270 | pBOB-N-Flag-GK_v1 | Lentivector encoding Flag-tagged GK (isoform 1) |
| 134271 | pBOB-N-Flag-GK_v2 | Lentivector encoding Flag-tagged GK (isoform 2) |
| 134272 | pBOB-N-Flag-GK (12-272) | Lentivector encoding Flag-tagged GK truncation (amino acids 12-272) |
| 134273 | pBOB-N-Flag-GK (281-473) | Lentivector encoding Flag-tagged GK truncation (amino acids 281-473) |
| 134275 | pBOB-N-3HA-GK_v1 | Lentivector encoding 3XHA-tagged GK (isoform 1) |
| 134277 | pBOB-N-3HA-GK_v2 | Lentivector encoding 3XHA-tagged GK (isoform 2) |
| 134279 | pcDNA6-GK5_v1 | Expresses GK5 (isoform 1) in mammalian cells |
| 134280 | pcDNA6-GK5_v2 | Expresses GK5 (isoform 2) in mammalian cells |
| 134281 | pcDNA6-C-Flag-GK5_v1 | Expresses Flag-tagged GK5 (isoform 1) in mammalian cells |
| 134282 | pcDNA6-C-Flag-GK5_v2 | Expresses Flag-tagged GK5 (isoform 2) in mammalian cells |
| 134283 | pcDNA6-N-Flag-SREBP1 | Expresses Flag-tagged SREBP1 in mammalian cells |
| 134284 | pcDNA6-N-Flag-SREBP2 | Expresses Flag-tagged SREBP2 in mammalian cells |
| 134285 | pcDNA6-N-Myc-SCAP | Expresses Myc-tagged SCAP in mammalian cells |
| 134286 | pLV-puro-N-3HA-SREBP1-C-Flag | Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1 |
| 134287 | pLV-puro-N-3HA-SREBP2-C-Flag | Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2 |
| 134288 | pMBP-parallel1-GK5_v2 | Expresses GK5 (isoform 2) in bacteria |
| 134289 | pMBP-parallel1-GK5_v2 (D30N) | Expresses GK5 (isoform 2)-D30N mutant in bacteria |
| 134290 | pMBP-parallel1-GK5_v2 (D280N) | Expresses GK5 (isoform 2)-D280N mutant in bacteria |
| 134291 | pMBP-parallel1-GK5_v2 (D30N+D280N+D443N) | Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria |
| 134292 | pMBP-parallel1-GK5_v2 (D30N+D280N) | Expresses GK5 (isoform 2)-D30N/D280N mutant in bacteria |
| 134293 | pMBP-parallel1-GK5_v2 (D443N) | Expresses GK5 (isoform 2)-D443N mutant in bacteria |