A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair.
Wen Y, Liao G, Pritchard T, Zhao TT, Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB
J Biol Chem. 2017 Apr 14;292(15):6148-6162. doi: 10.1074/jbc.M117.777722. Epub 2017 Feb 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133385 | BII-CPspaH2B | Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR) |
| 133386 | BII-C3H | Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR) |
| 133387 | BII-C3H2B | Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR) |
| 133388 | BII-C3HTK | Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR) |
| 133389 | BII-B3HTK | Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR) |
| 133390 | pBS-PtH2B | Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR) |
| 133391 | pBS-PtH | Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR) |
| 133392 | pBS-BtH | Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR) |