A photoactivatable Cre-loxP recombination system for optogenetic genome engineering.
Kawano F, Okazaki R, Yazawa M, Sato M
Nat Chem Biol. 2016 Dec;12(12):1059-1064. doi: 10.1038/nchembio.2205. Epub 2016 Oct 10.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
122960 | pcDNA3.1_PA-Cre | Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag) |
122961 | pcDNA3.1_PA-Cre-Y324F | Catalytically inactive mutant of PA-Cre |
122962 | pcDNA3.1_Floxed-STOP Fluc | Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction |
122963 | pcDNA3.1_Floxed-STOP mCherry | mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction |