The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12.
Shen H, Campanello GC, Flicker D, Grabarek Z, Hu J, Luo C, Banerjee R, Mootha VK
Cell. 2017 Nov 2;171(4):771-782.e11. doi: 10.1016/j.cell.2017.09.051. Epub 2017 Oct 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111288 | HsCLYBL(aa23-340)-His | bacterial expression |
| 111289 | HsCLYBL(aa23-340) D320N-His | bacterial expression |
| 111290 | HsCLYBL-FLAG | mammalian expression |
| 111291 | HsCLYBL(D320A)-FLAG | mammalian expression |
| 111292 | CaMCLC-His | bacterial expression |
| 111293 | His-PaIct | bacterial expression |
| 111294 | MsCLYBL_sgRNA1 | CRISPR KO murine CLYBL |
| 111295 | MsCLYBL_sgRNA6 | CRISPR KO murine CLYBL |
| 111296 | MsMUT_sgRNA1 | CRISPR KO murine MUT |
| 111297 | MsMUT_sgRNA3 | CRISPR KO murine MUT |
| 111298 | Neg. Ctrl_sgRNA | CRISPR KO murine neg. ctrl. |