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Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition.

Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, Beroukhim R, Ligon KL, Boehm JS, Hahn WC
Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
111181pDONR SMARCB1 var 2SMARCB1 variant 2 in pDONR223 backbone
111182pLXI_TRC401 SMARCB1 var 2Inducible vector (untagged) with SMARCB1 variant 2
111183pLXI_TRC401 LacZControl inducible vector with LacZ (V5 tagged)
111184pLXI_TRC403 LacZControl inducible vector with LacZ (V5 tagged)
111185pLXI_TRC403 SMARCB1 var 2Inducible vector (V5 tagged) with SMARCB1 variant 2
117068pXPR_BRD003 UBE2C guide 1single guide RNA targeting UBE2C; guide 1
117071pXPR_BRD003 UBE2C guide 2single guide RNA targeting UBE2C; guide 2
117072pXPR_BRD003 Luciferasesingle guide RNA targeting Luciferase
117073pXPR_BRD003 PSMB5 guide 1single guide RNA targeting PSMB5; guide 1
117074pXPR_BRD003 PSMB5 guide 2single guide RNA targeting PSMB5; guide 2

Antibodies from Article