CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway.
Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, Floor SN, Corn JE
Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0. Epub 2018 Jul 27.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111091 | pCR1063 | Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg |
| 111092 | pCR1068 | BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus. |
| 111093 | pCR1075 | Expresses eGFP donor in pUC19 |
| 111094 | pCR1265 | lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1 |
| 111096 | pCR1110 | Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068 |
| 111097 | pCR1161 | Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068 |
| 111098 | pCR1162 | Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068 |
| 111099 | pCR1163 | Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068 |
| 111100 | pCR1164 | Expresses FANCD2_-_10068140.23-P1P2 sgRNA in pCR1068 |
| 111101 | pCR1277 | Expresses RAD51D_-_33446794.23-P1P2 sgRNA in pCR1068 |
| 111102 | pCR1082 | Expresses FANCE_-_35420187.23-P1P2 sgRNA in pCR1068 |
| 111103 | pCR1135 | Expresses FANCE_-_35420147.23-P1P2 sgRNA in pCR1068 |
| 111104 | pCR1178 | Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068 |
| 111105 | pCR1224 | Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068 |
| 111106 | pCR1271 | Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068 |
| 111107 | pCR1272 | Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068 |
| 111109 | pCR1322 | Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068 |
| 111110 | pCR1118 | Expresses HELQ_+_84376961.23-P1P2 sgRNA in pCR1068 |
| 111111 | pCR1165 | Expresses HELQ_-_84376979.23-P1P2 sgRNA in pCR1068 |
| 111112 | pCR1273 | Expresses RAD51B_-_68286555.23-P1P2 sgRNA in pCR1068 |
| 111113 | pCR1274 | Expresses RAD51B_-_68286667.23-P1P2 sgRNA in pCR1068 |
| 111114 | pCR1275 | Expresses RAD51C_+_56770199.23-P1P2 sgRNA in pCR1068 |
| 111115 | pCR1276 | Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068 |
| 111116 | pCR1319 | Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068 |
| 111117 | pCR1320 | Expresses UBE2T_-_202311067.23-P1P2 sgRNA in pCR1068 |
| 111118 | pCR1299 | Expresses USP1_+_62903191.23-P1P2 sgRNA in pCR1068 |
| 111119 | pCR1300 | Expresses USP1_+_62903144.23-P1P2 sgRNA in pCR1068 |
| 111120 | pCR1324 | Expresses WDR48_+_39093886.23-P1P2 sgRNA in pCR1068 |
| 111121 | pCR1278 | Expresses XRCC2_-_152373174.23-P1P2 sgRNA in pCR1068 |
| 111122 | pCR1279 | Expresses XRCC2_+_152373143.23-P1P2 sgRNA in pCR1068 |
| 111123 | pCR1280 | Expresses XRCC3_+_104181759.23-P1P2 sgRNA in pCR1068 |
| 111124 | pCR1281 | Expresses XRCC3_+_104181786.23-P1P2 sgRNA in pCR1068 |
| 111125 | pCR1323 | Expresses non-targeting_01224 sgRNA in pCR1068 |
| 111126 | pCR1316 | Expresses FANCA. |
| 111128 | pCR1306 | Expresses FANCE. |
| 111129 | pCR1446 | Expresses FANCF |
| 111131 | pCR1326 | Expresses UBE2T. |
| 111132 | pCR1325 | Expresses USP1. |
| 111133 | pCR1307 | Expresses WDR48. |