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Erasure of Tet-Oxidized 5-Methylcytosine by a SRAP Nuclease.

Kweon SM, Zhu B, Chen Y, Aravind L, Xu SY, Feldman DE
Cell Rep. 2017 Oct 10;21(2):482-494. doi: 10.1016/j.celrep.2017.09.055. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
84524pET30a-SRAPExpresses murine SRAP domain (250 aa) in bacteria
84567p3XFlag-YedKExpresses YedK in mammalian cells
84568pET30a-SRAP-delCys2Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2
86884pET30a-YedKBacterial expression of E. coli SRAP ortholog YedK
89691pGEX4T-1 Srap1Expresses murine Srap1 in bacteria

Antibodies from Article