Switch-like responses of two cholesterol sensors do not require protein oligomerization in membranes.

Gay A, Rye D, Radhakrishnan A
Biophys J. 2015 Mar 24;108(6):1459-69. doi: 10.1016/j.bpj.2015.02.008. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
111026	pALOD4	Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
111027	pALOD4 non-binding mutant	Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)