Optimized base editors enable efficient editing in cells, organoids and mice.
Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY, Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow LE
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110835 | pCMV-BE3-2X | CMV expression vector for BE3-2X construct (not codon optimized) |
| 110836 | pCMV-BE3-FNLS | CMV expression vector for BE3-FNLS construct (not codon optimized) |
| 110837 | pLenti-Cas9-P2A-Puro | Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized) |
| 110838 | pLenti-BE3-P2A-Puro | Lentiviral vector for constitutive expression of BE3 (not codon optimized) |
| 110839 | pLenti-BE3RA-P2A-Puro | Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) |
| 110840 | pLenti-2X-P2A-Puro | Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized) |
| 110841 | pLenti-FNLS-P2A-Puro | Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) |
| 110842 | pLenti-BE3-PGK-Puro | Lentiviral vector for constitutive expression of BE3 (not codon optimized) |
| 110843 | pLenti-BE3RA-PGK-Puro | Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) |
| 110844 | pLenti-FNLS-PGK-Puro | Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) |
| 110845 | pLenti-TRE3G-BE3-PGK-Puro | Lentiviral vector for dox-inducible expression of BE3 (not codon optimized) |
| 110846 | pLenti-TRE3G-BE3RA-PGK-Puro | Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized) |
| 110847 | pLenti-TRE3G-FNLS-PGK-Puro | Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized) |
| 110848 | pLenti-VQR-P2A-Puro | Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized) |
| 110849 | pLenti-VRER-P2A-Puro | Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized) |
| 110850 | pLenti-HF1-P2A-Puro | Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized) |
| 110851 | pLenti-VQRRA-P2A-Puro | Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized) |
| 110852 | pLenti-VRERRA-P2A-Puro | Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized) |
| 110853 | pLenti-HF1RA-P2A-Puro | Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized) |
| 110854 | pLenti-U6-tdTomato-P2A-BlasR (LRT2B) | Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers |
| 110855 | pLenti-VQR-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized) |
| 110856 | pLenti-VRER-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized) |
| 110857 | pLenti-HF1-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized) |
| 110858 | pLenti-VQRRA-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized) |
| 110859 | pLenti-VRERRA-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized) |
| 110860 | pLenti-HF1RA-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized) |
| 110861 | pLenti-VQR-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized) |
| 110862 | pLenti-VRER-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized) |
| 110863 | pLenti-HF1-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized) |
| 110864 | pLenti-VQRRA-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized) |
| 110865 | pLenti-VRERRA-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized) |
| 110866 | pLenti-HF1RA-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized) |
| 110867 | pLenti-BE3-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized) |
| 110868 | pLenti-BE3RA-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized) |
| 110869 | pLenti-FNLS-P2A-GFP-PGK-Puro | Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized) |
| 110870 | pLenti-xBE3-P2A-Puro | Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells |
| 110871 | pLenti-xCas9RA-P2A-Puro | Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized) |
| 110872 | pLenti-xFNLS-P2A-Puro | Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) |
| 110873 | pLenti-xF2X-P2A-Puro | Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) |
| 112671 | pCMV-BE3-FNLS(RA) | CMV expression vector for BE3-FNLS construct (codon optimized) |
| 112672 | pLenti-BE4RA-P2A-Puro | Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized) |
| 112673 | pLenti-BE4GamRA-P2A-Puro | Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized) |
| 112675 | pLenti-ABERA-P2A-Puro | Lentivirus for constitutive expression of ABE7.10 RA (codon optimized) |
| 112676 | pLenti-xBE4Gam-P2A-Puro | Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized) |