Escherichia coli dihydrofolate reductase catalyzed proton and hydride transfers: temporal order and the roles of Asp27 and Tyr100.
Liu CT, Francis K, Layfield JP, Huang X, Hammes-Schiffer S, Kohen A, Benkovic SJ
Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):18231-6. doi: 10.1073/pnas.1415940111. Epub 2014 Dec 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110826 | pET22b-ecDHFR-Y100F | Bacterial expression of E.coli DHFR Y100F mutant |
| 110827 | pET22b-ecDHFR-D27S/Y100F | Bacterial expression of E.coli DHFR D27S/Y100F mutant |