An enhanced CRISPR repressor for targeted mammalian gene regulation.
Yeo NC, Chavez A, Lance-Byrne A, Chan Y, Menn D, Milanova D, Kuo CC, Guo X, Sharma S, Tung A, Cecchi RJ, Tuttle M, Pradhan S, Lim ET, Davidsohn N, Ebrahimkhani MR, Collins JJ, Lewis NE, Kiani S, Church GM
Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 10.1038/s41592-018-0048-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110820 | dCas9-KRAB | Conventional dCas9 repressor-dCas9-KRAB |
| 110821 | dCas9-KRAB-MeCP2 | Improved dCas9 repressor-dCas9-KRAB-MeCP2 |
| 110822 | pB-CAGGS-dCas9-KRAB | PiggyBac compatible plasmid expressing dCas9-KRAB |
| 110823 | pB-CAGGS-dCas9 | PiggyBac compatible plasmid expressing dCas9 |
| 110824 | pB-CAGGS-dCas9-KRAB-MeCP2 | PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 |