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A Calmodulin C-Lobe Ca(2+)-Dependent Switch Governs Kv7 Channel Function.

Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL Jr.
Neuron. 2018 Feb 21;97(4):836-852.e6. doi: 10.1016/j.neuron.2018.01.035. Epub 2018 Feb 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
111052Kv7.4 AB (325-557, Δ 368-523)/pET28HMTProtein expression in E.Coli
111072Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMTProtein expression in E.Coli
111073Kv7.4 BD (522-645, C643A)/pET28HMTProtein expression in E.Coli
111267Kv7.5 AB (361-545, Δ395-511)/pET28HMTProtein expression in E.Coli
111398Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMTProtein expression in E.Coli
111399Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMTProtein expression in E.Coli
111400Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMTProtein expression in E.Coli
111451Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMTProtein expression in E.Coli
111452Kv7.1/pcDNA3.1Electrophysiology in HEK cell
111453Kv7.4 (1-695)/pcDNA3.1Electrophysiology in HEK cell
111454Kv7.4 (1-645, Δ368-492)/pcDNA3.1Electrophysiology in HEK cell
111456Kv7.4 (1-645, Δ368-523)/pcDNA3.1Electrophysiology in HEK cell
111459Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1Electrophysiology in HEK cell
111460Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1Electrophysiology in HEK cell
111461Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1Electrophysiology in HEK cell
111462Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1Electrophysiology in HEK cell
111497Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1Electrophysiology in HEK cell
111498Kv7.5/pcDNA3.1Electrophysiology in HEK cell
111499CaM/pIRES2-eGFPElectrophysiology in HEK cell
111512CaM12(D20A/D56A)/pIRES2-eGFPElectrophysiology in HEK cell
111517CaM34(D93A/D129A)/pIRES2-eGFPElectrophysiology in HEK cell
111518CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFPElectrophysiology in HEK cell

Antibodies from Article