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Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation.

Zeng H, Jorapur A, Shain AH, Lang UE, Torres R, Zhang Y, McNeal AS, Botton T, Lin J, Donne M, Bastian IN, Yu R, North JP, Pincus L, Ruben BS, Joseph NM, Yeh I, Bastian BC, Judson RL
Cancer Cell. 2018 Jul 9;34(1):56-68.e9. doi: 10.1016/j.ccell.2018.05.014. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
110728pHIV-INK4A-zsGreenMammalian Expression of p16INK4A IRES zsGreen
110729pHIV-ARF-zsGreenMammalian Expression of p14ARF IRES zsGreen
110730pHIV-INK4A-mOrange2Mammalian Expression of p16INK4A IRES H2B-mOrange2
110731pHIV-ARF-mOrange2Mammalian Expression of p14ARF IRES H2B-mOrange2
110732pHIV-BRAFV600E-mOrange2Mammalian Expression of BRAFV600E IRES H2B-mOrange2
110733pGL410-BRN2pLuciferase reporter for the human BRN2 promoter
110734pHDR-CDKN2A-Ex2-CMV-EGFPRecombination template for replacing CDKN2A Exon2 with CMV-EGFP
110735pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2
110736pEN35-CDKN2A-Ex2-L Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2
110737pEN35-CDKN2A-Ex2-R Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

Antibodies from Article