Bioluminescent Low-Affinity Ca(2+) Indicator for ER with Multicolor Calcium Imaging in Single Living Cells.
Hossain MN, Suzuki K, Iwano M, Matsuda T, Nagai T
ACS Chem Biol. 2018 Mar 9. doi: 10.1021/acschembio.7b01014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111922 | CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3 | Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ |
| 111923 | CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3 | Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ |
| 111924 | CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3 | Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ |
| 111925 | Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3 | Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ |
| 111926 | CeNL(Ca2+)_1.2μ-pcDNA3 | Cyan color bright luminescent calcium indicator with intermediate affinity |
| 111927 | CeNL(Ca2+)_19μ-pcDNA3 | Cyan color bright luminescent calcium indicator with intermediate affinity |
| 111928 | OeNL(Ca2+)_18μ-pcDNA3 | Orange color bright luminescent calcium indicator with intermediate affinity |
| 111929 | CeNL(Ca2+)_110μ-pcDNA3 | Cyan color bright luminescent calcium indicator with low affinity |