Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.
Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, Ashworth A, Lord CJ
Nat Commun. 2018 May 22;9(1):2016. doi: 10.1038/s41467-018-04466-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110646 | pBiFC-PBZ-VN | expresses a PAR-binding (PBZ) domain fused to venus N-terminus |
| 110647 | pBiFC-PBZ-4A-VN | expresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding |
| 110648 | pBiFC-PBZ-VC | expresses a PAR-binding (PBZ) domain fused to venus C-terminus |
| 110649 | pBiFC-PBZ-4A-VC | expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding |
| 110650 | pcDNA3-PBZ-mRuby2 | expresses a PAR-binding (PBZ) domain fused to mRuby2 |
| 110651 | UPATrap-VC-ORF0 | transposon containing BiFC VC cassette |
| 110652 | UPATrap-VC-ORF1 | transposon containing BiFC VC cassette |
| 110653 | UPATrap-VC-ORF2 | transposon containing BiFC VC cassette |