Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids.
Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE
ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110573 | pBTK001 | Broad-host-range toolkit (BTK) entry vector, with superfolder GFP |
| 110574 | pBTK102 | BTK Type 2 promoter for golden gate assembly |
| 110575 | pBTK107 | BTK Type 2 promoter for golden gate assembly |
| 110576 | pBTK110 | BTK Type 2 promoter for golden gate assembly |
| 110577 | pBTK112 | BTK Type 2 promoter for golden gate assembly |
| 110578 | pBTK113 | BTK Type 2 promoter for golden gate assembly |
| 110581 | pBTK121 | BTK Type 2 promoter for golden gate assembly |
| 110582 | pBTK103 | BTK Type 2 promoter for golden gate assembly |
| 110583 | pBTK124 | BTK Type 2 promoter for golden gate assembly |
| 110584 | pBTK130 | BTK Type 2 promoter for golden gate assembly |
| 110587 | pBTK205 | BTK Type 3 coding sequence for golden gate assembly |
| 110588 | pBTK206 | BTK Type 3 coding sequence for golden gate assembly |
| 110590 | pBTK224 | BTK Type 3 coding sequence for golden gate assembly |
| 110591 | pBTK229 | BTK Type 3 coding sequence for golden gate assembly and homologous recombination |
| 110592 | pBTK300 | BTK Type 4 terminator for golden gate assembly |
| 110593 | pBTK301 | BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts) |
| 110594 | pBTK305 | BTK Type 4 terminator for golden gate assembly |
| 110595 | pBTK211 | BTK Type 6 Repressor CDS for golden gate assembly |
| 110597 | pBTK401 | BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly |
| 110598 | pBTK402 | BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly |
| 110599 | pBTK403 | BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly |
| 110600 | pBTK599 | BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination |
| 110601 | pBTK599s | BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination |
| 110602 | pBTK501 | BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance) |
| 110603 | pBTK519 | BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance) |
| 110604 | pBTK520 | BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance) |
| 110605 | pBTK527 | BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance) |
| 110606 | pBTK527a | BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance) |
| 110607 | pBTK601 | BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration) |
| 110608 | pBTK614 | BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes) |
| 110609 | pBTK615 | BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9 |
| 110610 | pBTK509 | BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter |
| 110611 | pBTK510 | BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter |
| 110612 | pBTK563 | BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter |
| 110613 | pBTK564 | BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter |
| 110614 | pBTK569 | BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter |
| 110615 | pBTK570 | BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter |
| 110616 | pBTK503 | BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter |
| 110617 | pBTK550d | BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase |
| 110618 | pBTK552 | BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI. |
| 110619 | pBTK619 | BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter |
| 110620 | pBTK620 | BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter |
| 110621 | pBTK621 | BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter |