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The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System Allows Tightly Controlled Gene Expression over a Wide Range in Pseudomonas aeruginosa.

Meisner J, Goldberg JB
Appl Environ Microbiol. 2016 Oct 27;82(22):6715-6727. doi: 10.1128/AEM.02041-16. Print 2016 Nov 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
110554pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)miniTn7 delivery plasmid with araC-ParaBAD inducible promoter
110558pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)miniTn7 delivery plasmid with lacIq-Ptac inducible promoter
110559pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter
110560pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert
110561pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert
110567pJM251 (miniCTX1-araC-ParaBAD)Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter
110571pJM253 (miniCTX1-rhaSR-PrhaBAD)Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter
110572pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Antibodies from Article