Probing the electrostatics of active site microenvironments along the catalytic cycle for Escherichia coli dihydrofolate reductase.
Liu CT, Layfield JP, Stewart RJ 3rd, French JB, Hanoian P, Asbury JB, Hammes-Schiffer S, Benkovic SJ
J Am Chem Soc. 2014 Jul 23;136(29):10349-60. doi: 10.1021/ja5038947. Epub 2014 Jul 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110541 | pET22b-ecDHFR-dCys | Bacterial protein of E.coli DHFR C85A/C152S mutant |
| 110542 | pET22b-ecDHFR-dCys-L54C | Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant |
| 110543 | pET22b-ecDHFR-dCys-T46C | Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant |