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Sandra Martha Gomes Dias Lab: Dias lab diverse plasmid collection

Unpublished

Plasmids from Article

ID Plasmid Purpose
110417pcDNA3.1D V5-His-TOPO-HuR.wtMammalian expression of HuR (wild type)
110419pLKO.1-blast shGFPshGFP control, silence GFP gene, blasticidin selection.
110420pLKO.1-blast shKGAshKGA, silence glutaminase KGA isoform, blasticidin selection.
110421tet-pLKO.puro_shGFPshGFP control, silence GFP gene, doxycycline inducible, puromycin selection
110422tet-pLKO.puro_shLucshLuc control, silence Luc gene, doxycycline inducible, puromycin selection
110423tet-pLKO.puro_shGACshGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection
110426pLKO.1-TRC.mKO2_shHuR CDSTRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.

Antibodies from Article