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Guanylate-binding protein-1 is a potential new therapeutic target for triple-negative breast cancer.

Quintero M, Adamoski D, Reis LMD, Ascencao CFR, Oliveira KRS, Goncalves KA, Dias MM, Carazzolle MF, Dias SMG
BMC Cancer. 2017 Nov 7;17(1):727. doi: 10.1186/s12885-017-3726-2. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
85208pLKO.1-TRC.mKO2Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression
85209pLKO.1-shGBP1.1.mKO2TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
85210pLKO.1-shGBP1.2.mKO2TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
85224pLKO.1-shLuc.mKO2shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.
110318pLKO.1-TRC.mKO2_shGFPControl (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2
110319pLKO.1-TRC.mKO2_shGLSshGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2
110320pLKO.1-TRC.mKO2_shARL4C.1TRCN0000048179 (Target GTCCCTGCATATCGTCATGTT), silence human ARL4C gene and express monomeric Kusabira-Orange2
110323pLKO.1-TRC.mKO2_shHRASLS.1TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2
110324pLKO.1-TRC.mKO2_shHRASLS.2TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2
110325pLKO.1-TRC.mKO2_shB3GNT5.1TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

Antibodies from Article