Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled DNA-Protein Crosslink Repair.
Vaz B, Popovic M, Newman JA, Fielden J, Aitkenhead H, Halder S, Singh AN, Vendrell I, Fischer R, Torrecilla I, Drobnitzky N, Freire R, Amor DJ, Lockhart PJ, Kessler BM, McKenna GW, Gileadi O, Ramadan K
Mol Cell. 2016 Nov 17;64(4):704-719. doi: 10.1016/j.molcel.2016.09.032. Epub 2016 Oct 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110216 | pCDNA3-Flag-SPRTN-Y117C | Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus. |
| 110217 | pNIC-ZB-SPRTNwt-FL | Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus. |
| 110218 | pNIC-ZB-SPRTN-E112A-FL | Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus. |
| 110219 | pNIC-ZB-SPRTN-Y117C-FL | Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus. |