Evaluation of 244,000 synthetic sequences reveals design principles to optimize translation in Escherichia coli.
Cambray G, Guimaraes JC, Arkin AP
Nat Biotechnol. 2018 Nov;36(10):1005-1015. doi: 10.1038/nbt.4238. Epub 2018 Sep 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110206 | pGC4593 | Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF) |
| 113408 | pGC4780 | Translation reporter with inducible translation coupling (high native GFP expression) |
| 113409 | pGC4785 | Translation reporter with inducible translation coupling (low native GFP expression, high under coupling) |