Systematic Dissection of Sequence Elements Controlling sigma70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli.

Urtecho G, Tripp AD, Insigne K, Kim H, Kosuri S
Biochemistry. 2018 Feb 1. doi: 10.1021/acs.biochem.7b01069. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
106250	pLibacceptorV2	Recombination-mediated cassette exchange with target genomic loci
110241	E. coli K-12 MG1655_rLP1(atpI-gidB)	E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
110242	E. coli K-12 MG1655_fLP2(yieN-trkD)	E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
110243	E. coli K-12 MG1655_fLP3(ybbD-ylbG)	E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
110244	E. coli K-12 MG1655_fLP4(essQ-cspB)	E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
110245	E. coli K-12 MG1655_rLP5(nth-ydgR)	E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.
110246	E. coli K-12 MG1655_rLP6(ygcE-ygcF)	E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.