Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling.
Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J
Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110164 | pX458_Ruby | Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA |
| 120816 | pcDNA3.2/Capsid | Ectopic expression of ZIKV protein |
| 120817 | pcDNA3.2/pr-Membrane | Ectopic expression of ZIKV protein |
| 120818 | pcDNA3.2/NS2A | Ectopic expression of ZIKV protein |
| 120819 | pcDNA3.2/NS2B | Ectopic expression of ZIKV protein |
| 120820 | pcDNA3.2/NS4A-2K | Ectopic expression of ZIKV protein |
| 120821 | pcDNA3.2/Envelope | Ectopic expression of ZIKV protein |
| 120822 | pcDNA3.2/NS3 | Ectopic expression of ZIKV protein |
| 120823 | pcDNA3.2/NS4B | Ectopic expression of ZIKV protein |
| 120824 | pcDNA3.2/NS1 | Ectopic expression of ZIKV protein |
| 120825 | pcDNA3.2/NS5 | Ectopic expression of ZIKV protein |
| 120826 | pcDNA3.2/NS4A | Ectopic expression of ZIKV protein |
| 120827 | pcDNA3.2/2K-NS4B | Ectopic expression of ZIKV protein |
| 120828 | pcDNA3.2/NS4A-2K-NS4B | Ectopic expression of ZIKV protein |
| 120829 | pcDNA3.2/NS5-Af-mut | Ectopic expression of ZIKV protein |
| 120830 | pcDNA3.2/NS5-Mt-mut | Ectopic expression of ZIKV protein |
| 120831 | pcDNA3.2/NS5-YFP | Ectopic expression of ZIKV protein |
| 120832 | pcDNA3.2/NS5-YFP-NLS-mut | Ectopic expression of ZIKV protein |
| 120833 | pcDNA3.2/EV | Control vector, 20nt random sequence between gateway recombination sites |