A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs.
Huang C, Liang D, Tatomer DC, Wilusz JE
Genes Dev. 2018 May 17. pii: gad.314856.118. doi: 10.1101/gad.314856.118.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110113 | Hy_pMT dati Exons 1-3 | Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA |
| 110114 | Hy_pMT dati MCS circfirefly_500 | Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells |
| 110115 | Hy_pMT dati MCS circfirefly_700 | Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells |
| 110116 | Hy_pMT dati MCS circfirefly_900 | Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells |
| 110117 | Hy_pMT dati MCS circfirefly_1100 | Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells |
| 110118 | Hy_pMT dati MCS circfirefly_1677 | Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells |
| 110119 | Hy_pMT dati MCS Exon | Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing |
| 110120 | Hy_pMT Flag-Hel25E D193E | Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity |
| 110121 | Hy_pMT Flag-Hel25E K91N | Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity |
| 110122 | Hy_pMT Flag-Hel25E MUT | Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity |
| 110123 | Hy_pMT Flag-Hel25E WT | Expresses Flag-tagged D. melanogaster Hel25E |
| 110124 | Hy_pMT Flag-UAP56 MUT | Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity |
| 110125 | Hy_pMT Flag-UAP56 WT | Expresses Flag-tagged human UAP56 (DDX39B) |
| 110126 | Hy_pMT Flag-URH49 MUT | Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity |
| 110127 | Hy_pMT Flag-URH49 WT | Expresses Flag-tagged human URH49 (DDX39A) |