A uniform cloning platform for mycobacterial genetics and protein production.
Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, Sander P, Hilbi H, Seeger MA
Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110074 | pMINTNH3 | Integrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage site |
| 110075 | pMINTC3H | Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag. |
| 110076 | pMINTC3GH (Kan) | Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion. |
| 110077 | pMINTC3GH (Hyg) | Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion. |
| 110078 | pMINTC3GH (Apr) | Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion. |
| 110079 | pMEXNH3 | Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria. |
| 110080 | pMEXC3H | Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria. |
| 110081 | pMEXC3GH (Kan) | Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria. |
| 110082 | pMEXC3GH (Hyg) | Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria. |
| 110083 | pMEXC3GH (Apr) | Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria. |
| 110084 | pACEC3GH (Hyg) | Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins. |
| 110085 | pACEC3GH (Apr) | Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins. |
| 110086 | pKO (Kan) | Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection. |
| 110087 | pKO (Hyg) | Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection. |
| 110088 | pKO (Apr) | Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection. |
| 110089 | pKOdeltagalK (Kan) | Analogous to pKO but without the negative selection gene galK. |
| 110090 | pKOdeltagalK (Hyg) | Analogous to pKO but without the negative selection gene galK. |
| 110091 | pKOdeltagalK (Apr) | Analogous to pKO but without the negative selection gene galK. |
| 110092 | pKOdeltasacB (Kan) | Analogous to pKO but without the negative selection gene sacB. |
| 110093 | pCOND (Hyg) | Suicide vector based on the TetR/Pip OFF system. |
| 110094 | pCOND (Apr) | Suicide vector based on the TetR/Pip OFF system. |
| 110095 | pFLAG_attP | Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting. |
| 110096 | pMA_Int | E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation. |
| 110097 | pFLAG_OriM | Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector. |