A versatile nanobody-based toolkit to analyze retrograde transport from the cell surface.
Buser DP, Schleicher KD, Prescianotto-Baschong C, Spiess M
Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):E6227-E6236. doi: 10.1073/pnas.1801865115. Epub 2018 Jun 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109417 | pET24a-VHH-std | Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope |
| 109418 | pET24a-VHH-1xTS | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope |
| 109419 | pET24a-VHH-2xTS | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope |
| 109420 | pET24a-VHH-APEX2 | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope |
| 109421 | pET24a-VHH-mCherry | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope |
| 109422 | pET24a-VHH-myc | Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope |
| 109423 | pET24a-VHH-tev-std | Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope |
| 109424 | pET21d-myc-BirA | Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope |