A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.
Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP
Science. 2017 Feb 10;355(6325). pii: 355/6325/eaai8386. doi: 10.1126/science.aai8386.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110168 | pET29b-PD | Expresses propanediol dehydratase in E. coli BL21(DE3) |
| 110174 | pET29b-PD-G817A | Expresses propanediol dehydratase mutant G817A in E. coli BL21(DE3) |
| 110177 | pET29b-PD-C438S | Expresses propanediol dehydratase mutant C438S in E. coli BL21(DE3) |
| 110178 | pET29b-PD-E440Q | Expresses propanediol dehydratase mutant E440Q in E. coli BL21(DE3) |
| 110179 | pET29b-PD-H166Q | Expresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3) |
| 110180 | pET28a-PDAE | Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3) |
| 110288 | pET28a-HypD | Expresses t-4-hydroxy-L-proline dehydratase in E. coli BL21(DE3) |
| 110295 | pET28a-P5CR | Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3) |
| 110296 | pSV272-PfMBP-HypDAE | Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3) |