Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize.
Lee K, Zhang Y, Kleinstiver BP, Guo JA, Aryee MJ, Miller J, Malzahn A, Zarecor S, Lawrence-Dill CJ, Joung JK, Qi Y, Wang K
Plant Biotechnol J. 2018 Jul 4. doi: 10.1111/pbi.12982.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109327 | pYPQ165 | Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter |
| 109328 | pYPQ166 | Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter |
| 109329 | pYPQ210 | Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection |