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A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing.

Li A, Lee CM, Hurley AE, Jarrett KE, De Giorgi M, Lu W, Balderrama KS, Doerfler AM, Deshmukh H, Ray A, Bao G, Lagor WR
Mol Ther Methods Clin Dev. 2018 Dec 6;12:111-122. doi: 10.1016/j.omtm.2018.11.009. eCollection 2019 Mar 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
1093141313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spAPlasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA
1093151476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spAPlasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp
1093161518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spAPlasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP
1093171530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spAPlasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA
1093181531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spAPlasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

Antibodies from Article