Addgene: A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing.

A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing.

Li A, Lee CM, Hurley AE, Jarrett KE, De Giorgi M, Lu W, Balderrama KS, Doerfler AM, Deshmukh H, Ray A, Bao G, Lagor WR
Mol Ther Methods Clin Dev. 2018 Dec 6;12:111-122. doi: 10.1016/j.omtm.2018.11.009. eCollection 2019 Mar 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
109318	1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA	Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9
109317	1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA	Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA
109316	1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA	Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP
109315	1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA	Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp
109314	1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA	Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA
109313	1162_pAAV-HLP-EmGFP-SpA	Plasmid for AAV EmGFP Mammalian Expression

Antibodies from Article