A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing.
Li A, Lee CM, Hurley AE, Jarrett KE, De Giorgi M, Lu W, Balderrama KS, Doerfler AM, Deshmukh H, Ray A, Bao G, Lagor WR
Mol Ther Methods Clin Dev. 2018 Dec 6;12:111-122. doi: 10.1016/j.omtm.2018.11.009. eCollection 2019 Mar 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109313 | 1162_pAAV-HLP-EmGFP-SpA | Plasmid for AAV EmGFP Mammalian Expression |
| 109314 | 1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA | Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA |
| 109315 | 1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA | Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp |
| 109316 | 1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA | Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP |
| 109317 | 1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA | Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA |
| 109318 | 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA | Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9 |