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Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages.

Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Ainsa JA, Hansen EJ, Gumbo T, van Oers NS
Appl Environ Microbiol. 2012 Oct;78(19):6829-37. doi: 10.1128/AEM.01674-12. Epub 2012 Jul 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
109285pSum-LacZ-gfpExpresses gfp in mycobacteria
109286pSUM-GroEL-gfpExpresses gfp in mycobacteria
109287pSUM-Kan-MCS3Expresses proteins in mycobacteria
109288pSUM-Kan-MCS1-gfpExpresses high levels of gfp in mycobacteria
109289pSUM-Kan-MCS2Expresses high levels of protein in mycobacteria
109290pSUM-Kan-MCS2-gfpExpresses high levels of gfp in mycobacteria
109296pCR2.1-promoter-mIL-6IL-6 promoter reporter construct
109379pSUM36-MCS2-mycShuttle vector for high level expression in mycobacteria.
112896pmIL-6promoterEGFPTo monitor the activation of the murine IL-6 promoter by eGFP expression

Antibodies from Article