Engineered promoters enable constant gene expression at any copy number in bacteria.
Segall-Shapiro TH, Sontag ED, Voigt CA
Nat Biotechnol. 2018 Mar 19. pii: nbt.4111. doi: 10.1038/nbt.4111.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109233 | pTHSSe_1 | Empty AmpR pSC101 vector |
| 109234 | pTHSSe_2 | Empty SpecR p15A vector |
| 109235 | pTHSSe_31 | PUPsp1 GFP reporter |
| 109236 | pTHSSe_32 | PUPsp2 GFP reporter |
| 109237 | pTHSSe_37 | PTac driving GFP |
| 109238 | pTHSSe_39 | PTac driving TALEsp1 |
| 109239 | pTHSSe_40 | PTac driving TALEsp2 |
| 109240 | pTHSSe_42 | pSC101 BbsI cloning vector |
| 109241 | pTHSSe_43 | pSC101 var1 BbsI cloning vector |
| 109242 | pTHSSe_44 | pSC101 var2 BbsI cloning vector |
| 109243 | pTHSSe_45 | pSC101 var3 BbsI cloning vector |
| 109244 | pTHSSe_46 | pSC101 var4 BbsI cloning vector |
| 109245 | pTHSSe_47 | pSC101 var5 BbsI cloning vector |
| 109246 | pTHSSe_48 | pSC101 var6 BbsI cloning vector |
| 109247 | pTHSSe_49 | pSC101 var7 BbsI cloning vector |
| 109248 | pTHSSe_50 | incW BbsI cloning vector |
| 109249 | pTHSSe_51 | p15A BbsI cloning vector |
| 109250 | pTHSSe_52 | ColE1 BbsI cloning vector |
| 109251 | pTHSSe_53 | pUC BbsI cloning vector |
| 109253 | pTHSSe_59 | TALEsp1 stabilized promoter driving GFP |
| 109254 | pTHSSe_60 | TALEsp2 stabilized promoter driving GFP |