Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase.
Schutsky EK, DeNizio JE, Hu P, Liu MY, Nabel CS, Fabyanic EB, Hwang Y, Bushman FD, Wu H, Kohli RM
Nat Biotechnol. 2018 Oct 8. pii: nbt.4204. doi: 10.1038/nbt.4204.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109231 | pET41-MBP-t-A3A-t-His | Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease |