Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis.
Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y
Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109030 | pET28b-NmCas9-Flag | Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression |
| 109031 | pET28b-NmCas9-Flag D16A | D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression |
| 109032 | pET28b-NmCas9-Flag H588A | H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression |
| 109033 | pET28b-NmCas9-Flag D16A+H588A | D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression |