Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.
Paschke P, Knecht DA, Silale A, Traynor D, Williams TD, Thomason PA, Insall RH, Chubb JR, Kay RR, Veltman DM
PLoS One. 2018 May 30;13(5):e0196809. doi: 10.1371/journal.pone.0196809. eCollection 2018.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108974 | pDM344 | shuttle vector |
| 108975 | pDM1018 | shuttle vector, N-terminal mCherry tagging |
| 108976 | pDM1019 | shuttle vector, N-terminal GFP tagging |
| 108977 | pDM1020 | shuttle vector, C-terminal mCherry tagging |
| 108978 | pDM1021 | shuttle vector, C-terminal GFP tagging |
| 108979 | pDM1079 | Knockout cassette Blasticidin |
| 108980 | pDM1080 | Knockout cassette Nourseothricin |
| 108981 | pDM1081 | Knockout cassette Hygromycin |
| 108982 | pDM1082 | Knockout cassette G418 |
| 108983 | pDM1203 | extrachromosomal expression vector |
| 108984 | pDM1207 | extrachromosomal expression vector, N-terminal GFP tagging |
| 108985 | pDM1208 | extrachromosomal expression vector, N-terminal mCherry tagging |
| 108986 | pDM1209 | extrachromosomal expression vector, C-terminal GFP tagging |
| 108987 | pDM1210 | extrachromosomal expression vector, C-terminal mCherry tagging |
| 108988 | pDM1038 | Inducible expression vector (dox-on) |
| 108989 | pDM1046 | Inducible expression vector (dox-on), N-terminal mCherry tagging |
| 108990 | pDM1047 | Inducible expression vector (dox-on), N-terminal GFP tagging |
| 108991 | pDM1048 | Inducible expression vector (dox-on), C-terminal mCherry tagging |
| 108992 | pDM1049 | Inducible expression vector (dox-on), C-terminal GFP tagging |
| 108993 | pDM1220 | integrating expression vector |
| 108994 | pDM1355 | generic knockin vector for C-terminal tagging with GFP |
| 108995 | pDM1488 | Cre expression vector |
| 108996 | pDM1489 | Cre expression vector |
| 108997 | pDM1501 | safe haven knock-in plasmid for the act5 locus |
| 108998 | pDM1513 | safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging |
| 108999 | pDM1514 | safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging |
| 109000 | pDM1515 | safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging |
| 109001 | pDM1516 | safe haven knock-in plasmid for the act5 locus, C-terminal mCherry tagging |
| 113195 | pPI437 | extrachromosomal expression vector, N-terminal mScarlet tagging |
| 113196 | pPI459 | extrachromosomal expression vector, C-terminal mScarlet tagging |
| 113199 | pPI418 | shuttle vector, N-terminal mScarlet tagging |
| 113200 | pPI457 | shuttle vector, C-terminal mScarlet tagging |
| 113203 | pPI452 | Inducible expression vector (dox-on) N-terminal mScarlet tagging |
| 113204 | pPI460 | Inducible expression vector (dox-on), C-terminal mScarlet tagging |
| 113207 | pPI468 | integrating expression vector, N-terminal mScarlet tagging |
| 113208 | pPI467 | integrating expression vector, C-terminal mScarlet tagging |
| 113211 | pPI419 | safe haven knock-in plasmid for the act5 locus, N-terminal mScarlet tagging |
| 113212 | pPI458 | safe haven knock-in plasmid for the act5 locus, C-terminal mScarlet tagging |
| 113215 | pPI461 | generic knockin vector for C-terminal tagging with mCherry |
| 113216 | pPI464 | generic knockin vector for C-terminal tagging with mScarlet |
| 113218 | pPI191 | generic knockin vector for C-terminal tagging with GFP |
| 113219 | pPI192 | generic knockin vector for C-terminal tagging with mCherry |
| 113221 | pPI184 | generic knockin vector for C-terminal tagging with mCherry |
| 113222 | pPI180 | generic knockin vector for C-terminal tagging with GFP |
| 113227 | pPI278 | extrachromosomal expression vector, expression of H2B-GFP |
| 113228 | pPI226 | safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry |
| 113229 | pPI138 | extrachromosomal expression vector, expression of LifeAct-GFP |
| 113230 | pPI420 | safe haven knock-in plasmid for the act5 locus, Expression of H2B-mCherry |
| 113231 | pPI227 | safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP |
| 113232 | pPI304 | extrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFP |
| 207427 | pPI143 | Extrachromosomal expression for mNeonGreen Fusions C-terminal |
| 207428 | pPI152 | Shuttle Vector for mNeonGreen Fusions |
| 207430 | pPI159 | Extrachromosomal expression for mNeonGreen Fusions N-terminal |
| 207431 | pPI230 | Safe haven knock-in plasmid for the act5 locus for C-terminal mNeonGreen fusions |
| 207432 | pPI231 | Safe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusions |
| 207433 | pPI450 | Inducible extrachromosomal overexpression vector - mNeonGreen - N-terminal |
| 207434 | pPI462 | Knock in plasmid for mNeonGreen fusions C-terminal |
| 207435 | pPI465 | REMI integrated Expression for mNeonGreen Fusions N-terminal |
| 207436 | pPI470 | Inducible extrachromosomal overexpression vector - mNeonGreen - C-terminal |
| 207437 | pPI471 | REMI integrated Expression for mNeonGreen Fusions C-terminal |