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Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites.

Zhong Z, Zhang Y, You Q, Tang X, Ren Q, Liu S, Yang L, Wang Y, Liu X, Liu B, Zhang T, Zheng X, Le Y, Zhang Y, Qi Y
Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
108859pYPQ239FnCpf1 Gateway entry plasmid
108860pYPQ230-RRLbCpf1 Gateway entry plasmid with G548R and K611R mutations
108861pYPQ230-RVRLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations
108862pYPQ239-RRFnCpf1 Gateway entry plasmid with N623R and K687R mutations
108863pYPQ239-RVRFnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations
108864pYPQ141-ZmUbi-RZ-FnFnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

Antibodies from Article