High-performance chemical- and light-inducible recombinases in mammalian cells and mice.
Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J, Wong WW
Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
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PubMed
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Article
Chemical-inducible dimerization (CIDs) domains are proteins that can be dimerized through addition of a small-molecule. Moreover, light-inducible dimerization (LIDs) domains also exist that dimerize in response to light. This set of plasmids contains three pairs of heterodimerizing CIDs and one pair of heterodimerizing LIDs. These include the rapalog-responsive FKBP/FRB, gibberellin-responsive GAI/GID1, abscisic acid-responsive PYL/ABI CIDs and blue-light responsive nMag/pMag domains. CIDs/LIDs can be used to recruit proteins with different activities to localize the response. Moreover, CIDs/LIDs can be used to restore the activity of a split protein. This latter strategy was used to develop a large suite of inducible split recombinases.
Supplemental Information
For more information please download the Wong Lab's Cloning Methods to Assemble Split Recombinase Plasmids
WilsonWong_Cloning_Methods.pdf 2.2Â MBPlasmid Pairs for Expressing Chemical and Light-inducible Recombinases
The following plasmid pairs are intended to be used together to produce light or chemically induced recombinase proteins.
Sub-Cloning Vectors For Creating Chemical or Light-inducible Proteins
A series of sub-cloning vectors containing CID or LID domains were assembled using standard molecular cloning techniques or Gibson isothermal assembly. For each CID or LID, two sub-cloning vectors were made such that a recombinase could be tagged with the domain on the C-terminus or N-terminus and a glycineserine rich linker. The cloning scheme is depicted in the image below. The backbone vectors are listed in the table underneath the figure and can be sorted by fusion protein or type of system.
The table below contains the full list of available plasmids from this article.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108723 | pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa229 of Cre recombinase fused to GID1 CID |
| 108724 | pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA | Expresses aa230 to C-terminus of Cre recombinase fused to GAI CID |
| 108725 | pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa251 of Cre recombinase fused to GID1 CID |
| 108726 | pBW2230_pCAG-PV1-GAI-L1-iCre-252C-NLS-BGHpA | Expresses aa252 to C-terminus of Cre recombinase fused to GAI CID |
| 108727 | pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa256 of Cre recombinase fused to GID1 CID |
| 108728 | pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpA | Expresses aa257 to C-terminus of Cre recombinase fused to GAI CID |
| 108729 | pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID |
| 108730 | pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA | Expresses aa271 to C-terminus of Cre recombinase fused to GAI CID |
| 108731 | pBW2598_pCAG-PV1-iCre-N229-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa229 of Cre recombinase fused to FRB CID |
| 108732 | pBW2606_pCAG-PV1-FKBP-L1-iCre-230C-NLS-BGHpA | Expresses aa230 to C-terminus of Cre recombinase fused to FKBP CID |
| 108733 | pBW2600_pCAG-PV1-iCre-N251-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa251 of Cre recombinase fused to FRB CID |
| 108734 | pBW2608_pCAG-PV1-FKBP-L1-iCre-252C-NLS-BGHpA | Expresses aa252 to C-terminus of Cre recombinase fused to FKBP CID |
| 108735 | pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID |
| 108736 | pBW2610_pCAG-PV1-FKBP-L1-iCre-257C-NLS-BGHpA | Expresses aa257 to C-terminus of Cre recombinase fused to FKBP CID |
| 108737 | pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID |
| 108738 | pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpA | Expresses aa271 to C-terminus of Cre recombinase fused to FKBP CID |
| 108739 | pBW2599_pCAG-PV1-iCre-N229-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa229 of Cre recombinase fused to ABI CID |
| 108740 | pBW2607_pCAG-PV1-PYL-L1-iCre-230C-NLS-BGHpA | Expresses aa230 to C-terminus of Cre recombinase fused to PYL CID |
| 108741 | pBW2601_pCAG-PV1-iCre-N251-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa251 of Cre recombinase fused to ABI CID |
| 108742 | pBW2609_pCAG-PV1-PYL-L1-iCre-252C-NLS-BGHpA | Expresses aa252 to C-terminus of Cre recombinase fused to PYL CID |
| 108743 | pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa256 of Cre recombinase fused to ABI CID |
| 108744 | pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA | Expresses aa257 to C-terminus of Cre recombinase fused to PYL CID |
| 108745 | pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa270 of Cre recombinase fused to ABI CID |
| 108746 | pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA | Expresses aa271 to C-terminus of Cre recombinase fused to PYL CID |
| 108747 | pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa27 of Flp recombinase fused to GID1 CID |
| 108748 | pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA | Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID |
| 108749 | pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa168 of Flp recombinase fused to GID1 CID |
| 108750 | pBW2184_pCAG-PV1-GAI-L1-FlpO-169C-NLS-BGHpA | Expresses aa169 to C-terminus of Flp recombinase fused to GAI CID |
| 108751 | pBW2173_pCAG-PV1-FlpO-N374-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa374 of Flp recombinase fused to GID1 CID |
| 108752 | pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA | Expresses aa375 to C-terminus of Flp recombinase fused to GAI CID |
| 108753 | pBW2175_pCAG-PV1-FlpO-N396-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa396 of Flp recombinase fused to GID1 CID |
| 108754 | pBW2195_pCAG-PV1-GAI-L1-FlpO-397C-NLS-BGHpA | Expresses aa397 to C-terminus of Flp recombinase fused to GAI CID |
| 108755 | pBW2272_pCAG-PV1-FlpO-N27-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa27 of Flp recombinase fused to FRB CID |
| 108756 | pBW2273_pCAG-PV1-FKBP-L1-FlpO-28C-NLS-BGHpA | Expresses aa28 to C-terminus of Flp recombinase fused to FKBP CID |
| 108757 | pBW2276_pCAG-PV1-FlpO-N168-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa168 of Flp recombinase fused to FRB CID |
| 108758 | pBW2277_pCAG-PV1-FKBP-L1-FlpO-169C-NLS-BGHpA | Expresses aa169 to C-terminus of Flp recombinase fused to FKBP CID |
| 108759 | pBW2280_pCAG-PV1-FlpO-N374-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa374 of Flp recombinase fused to FRB CID |
| 108760 | pBW2281_pCAG-PV1-FKBP-L1-FlpO-375C-NLS-BGHpA | Expresses aa375 to C-terminus of Flp recombinase fused to FKBP CID |
| 108761 | pBW2284_pCAG-PV1-FlpO-N396-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa396 of Flp recombinase fused to FRB CID |
| 108762 | pBW2285_pCAG-PV1-FKBP-L1-FlpO-397C-NLS-BGHpA | Expresses aa397 to C-terminus of Flp recombinase fused to FKBP CID |
| 108763 | pBW2274_pCAG-PV1-FlpO-N27-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa27 of Flp recombinase fused to ABI CID |
| 108764 | pBW2275_pCAG-PV1-PYL-L1-FlpO-28C-NLS-BGHpA | Expresses aa28 to C-terminus of Flp recombinase fused to PYL CID |
| 108765 | pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa168 of Flp recombinase fused to ABI CID |
| 108766 | pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA | Expresses aa169 to C-terminus of Flp recombinase fused to PYL CID |
| 108767 | pBW2282_pCAG-PV1-FlpO-N374-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa374 of Flp recombinase fused to ABI CID |
| 108768 | pBW2283_pCAG-PV1-PYL-L1-FlpO-375C-NLS-BGHpA | Expresses aa375 to C-terminus of Flp recombinase fused to PYL CID |
| 108769 | pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID |
| 108770 | pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA | Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID |
| 108771 | pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa172 of VCre recombinase fused to GID1 CID |
| 108772 | pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA | Expresses aa173 to C-terminus of VCre recombinase fused to GAI CID |
| 108773 | pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID |
| 108774 | pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA | Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID |
| 108775 | pBW2550_pCAG-PV1-VCre-N277-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa277of VCre recombinase fused to GID1 CID |
| 108776 | pBW2570_pCAG-PV1-GAI-L1-VCre-278C-NLS-BGHpA | Expresses aa278 to C-terminus of VCre recombinase fused to GAI CID |
| 108777 | pBW2582_pCAG-PV1-VCre-N172-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa172 of VCre recombinase fused to FRB CID |
| 108778 | pBW2586_pCAG-PV1-FKBP-L1-VCre-173C-NLS-BGHpA | Expresses aa173 to C-terminus of VCre recombinase fused to FKBP CID |
| 108779 | pBW2584_pCAG-PV1-VCre-N269-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa269 of VCre recombinase fused to FRB CID |
| 108780 | pBW2588_pCAG-PV1-FKBP-L1-VCre-270C-NLS-BGHpA | Expresses aa270 to C-terminus of VCre recombinase fused to FKBP CID |
| 108781 | pBW2618_pCAG-PV1-VCre-N277-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa277of VCre recombinase fused to FRB CID |
| 108782 | pBW2620_pCAG-PV1-FKBP-L1-VCre-278C-NLS-BGHpA | Expresses aa278 to C-terminus of VCre recombinase fused to FKBP CID |
| 108783 | pBW2583_pCAG-PV1-VCre-N172-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa172 of VCre recombinase fused to ABI CID |
| 108784 | pBW2587_pCAG-PV1-PYL-L1-VCre-173C-NLS-BGHpA | Expresses aa173 to C-terminus of VCre recombinase fused to PYL CID |
| 108785 | pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa269 of VCre recombinase fused to ABI CID |
| 108786 | pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA | Expresses aa270 to C-terminus of VCre recombinase fused to PYL CID |
| 108787 | pBW2619_pCAG-PV1-VCre-N277-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa277of VCre recombinase fused to ABI CID |
| 108788 | pBW2621_pCAG-PV1-PYL-L1-VCre-278C-NLS-BGHpA | Expresses aa278 to C-terminus of VCre recombinase fused to PYL CID |
| 108789 | pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID |
| 108790 | pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA | Expresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CID |
| 108791 | pBW1345_pCAG-PV1-PhiC31-N396-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa396 of PhiC31 integrase fused to FRB CID |
| 108792 | pBW1358_pCAG-PV1-FKBP-L1-PhiC31-397C-NLS-BGHpA | Expresses aa397 to C-terminus of PhiC31 integrase fused to FKBP CID |
| 108793 | pBW1346_pCAG-PV1-PhiC31-N428-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa428 of PhiC31 integrase fused to FRB CID |
| 108794 | pBW1359_pCAG-PV1-FKBP-L1-PhiC31-429C-NLS-BGHpA | Expresses aa429 to C-terminus of PhiC31 integrase fused to FKBP CID |
| 108795 | pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID |
| 108796 | pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA | Expresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID |
| 108797 | pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa233 of PhiC31 integrase fused to ABI CID |
| 108798 | pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA | Expresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID |
| 108799 | pBW1601_pCAG-PV1-PhiC31-N396-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa396 of PhiC31 integrase fused to ABI CID |
| 108800 | pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA | Expresses aa397 to C-terminus of PhiC31 integrase fused to PYL CID |
| 108801 | pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa428 of PhiC31 integrase fused to ABI CID |
| 108802 | pBW1607_pCAG-PV1-PYL-L1-PhiC31-429C-BGHpA | Expresses aa429 to C-terminus of PhiC31 integrase fused to PYL CID |
| 108803 | pBW1603_pCAG-PV1-PhiC31-N571-L1-ABI-NLS-BGHpA | Expresses N-terminus to aa571 of PhiC31 integrase fused to ABI CID |
| 108804 | pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA | Expresses aa572 to C-terminus of PhiC31 integrase fused to PYL CID |
| 108805 | pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID |
| 108806 | pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA | Expresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID |
| 108811 | pSD31_pCAG-PV1-TP901-N323-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa323 of TP901 integrase fused to GID1 CID |
| 108812 | pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpA | Expresses aa324 to C-terminus of TP901 integrase with N-term FKBP and C-term NLS |
| 108813 | pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA | Expresses N-terminus to aa326 of TP901 integrase fused to GID1 CID |
| 108814 | pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA | Expresses aa327 to C-terminus of TP901 integrase fused to FKBP |
| 108815 | pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA | Expresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CID |
| 108816 | pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA | Expresses aa469 to C-terminus of Bxb1 integrase fused to GAI CID |
| 108817 | pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa229 of Cre recombinase fused to nMag CID |
| 108818 | pBW2690_pCAG-PV1-pMag-L1-iCre-230C-BGHpA | Expresses aa230 to C-terminus of Cre recombinase fused to pMag CID |
| 108819 | pBW2693_pCAG-PV1-iCre-N251-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa251 of Cre recombinase fused to nMag CID |
| 108820 | pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA | Expresses aa252 to C-terminus of Cre recombinase fused to pMag CID |
| 108821 | pBW2697_pCAG-PV1-iCre-N256-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa256 of Cre recombinase fused to nMag CID |
| 108822 | pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA | Expresses aa257 to C-terminus of Cre recombinase fused to pMag CID |
| 108823 | pBW2701_pCAG-PV1-iCre-N270-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa270 of Cre recombinase fused to nMag CID |
| 108824 | pBW2702_pCAG-PV1-pMag-L1-iCre-271C-BGHpA | Expresses aa271 to C-terminus of Cre recombinase fused to pMag CID |
| 108825 | pBW2665_pCAG-PV1-FlpO-N27-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa27 of Flp recombinase fused to nMag CID |
| 108826 | pBW2666_pCAG-PV1-pMag-L1-FlpO-28C-NLS-BGHpA | Expresses aa28 to C-terminus of Flp recombinase fused to pMag CID |
| 108827 | pBW2669_pCAG-PV1-FlpO-N168-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa168 of Flp recombinase fused to nMag CID |
| 108828 | pBW2670_pCAG-PV1-pMag-L1-FlpO-169C-NLS-BGHpA | Expresses aa169 to C-terminus of Flp recombinase fused to pMag CID |
| 108829 | pBW2673_pCAG-PV1-FlpO-N374-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa374 of Flp recombinase fused to nMag CID |
| 108830 | pBW2674_pCAG-PV1-pMag-L1-FlpO-375C-NLS-BGHpA | Expresses aa375 to C-terminus of Flp recombinase fused to pMag CID |
| 108831 | pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA | Expresses N-terminus to aa396 of Flp recombinase fused to nMag CID |
| 108832 | pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpA | Expresses aa397 to C-terminus of Flp recombinase fused to pMag CID |
| 108833 | pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA | Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs |
| 108834 | pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminus |
| 108835 | pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus |
| 108836 | pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus |
| 108837 | pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus |
| 108838 | pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminus |
| 108839 | pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus |
| 108840 | pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus |
| 108841 | pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus |
| 108842 | pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus |
| 108843 | pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus |
| 108844 | pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminus |
| 108845 | pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus |
| 108846 | pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus |
| 108847 | pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus |
| 108848 | pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA | Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus |
| 108849 | pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA | Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus |