Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases.
Luna-Vargas MP, Christodoulou E, Alfieri A, van Dijk WJ, Stadnik M, Hibbert RG, Sahtoe DD, Clerici M, Marco VD, Littler D, Celie PH, Sixma TK, Perrakis A
J Struct Biol. 2011 Aug;175(2):113-9. doi: 10.1016/j.jsb.2011.03.017. Epub 2011 Mar 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108703 | pETNKI-his-3C-LIC-kan | Expression of an His-tagged target protein with 3C protease cleavage site. |
| 108704 | pETNKI-his-3C-LIC-Amp | Expression of a His-tagged target protein with 3C protease cleavage site. |
| 108705 | pCDFNKI-his-3C-LIC-strep | Expression of a His-tagged target protein with 3C protease cleavage site. |
| 108706 | pETNKI-strepII-3C-LIC-kan | Expression of a StrepII-tagged target protein with 3C protease cleavage site. |
| 108707 | pETNKI-strepII-3C-LIC-Amp | Expression of a StrepII-tagged target protein with 3C protease cleavage site. |
| 108708 | pCDFNKI-strepII-3C-LIC-strep | Expression of a StrepII-tagged target protein with 3C protease cleavage site. |
| 108709 | pGEXNKI-his-GST-3C-LIC | Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site. |
| 108710 | pGEXNKI-GST-3C-LIC | Expression of a GST-tagged target protein with 3C protease cleavage site. |