PCNA directs type 2 RNase H activity on DNA replication and repair substrates.
Bubeck D, Reijns MA, Graham SC, Astell KR, Jones EY, Jackson AP
Nucleic Acids Res. 2011 May;39(9):3652-66. doi: 10.1093/nar/gkq980. Epub 2011 Jan 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108695 | pGEX6P1‐Afu‐rnhB D101N | For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation |
| 108696 | pGEX6P1‐Afu‐rnhBΔPIP | For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion |
| 108697 | pEGFP‐RNASEH2B | For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells |
| 108698 | pEGFP‐RNASEH2C | For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells |
| 108699 | pEGFP‐RNASEH1 | For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells |
| 108700 | pEGFP‐RNASEH2A | For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells |