The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease.
Reijns MA, Bubeck D, Gibson LC, Graham SC, Baillie GS, Jones EY, Jackson AP
J Biol Chem. 2011 Mar 25;286(12):10530-9. doi: 10.1074/jbc.M110.177394. Epub 2010 Dec 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108692 | pGEX6P1‐hsRNASEH2BCA | For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme |
| 108693 | pGEX6P1‐hsRNASEH2BCA(D34A/D169A) | For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme |
| 108694 | pGEX6P1‐Afu rnhB | For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII |